- Title
- Genome-wide DNA methylation profiling of CD8+T cells shows a distinct epigenetic signature to CD4+T cells in multiple sclerosis patients
- Creator
- Maltby, Vicki E.; Graves, Moira C.; Lea, Rodney A.; Benton, Miles C.; Sanders, Katherine A.; Tajouri, Lotti; Scott, Rodney J.; Lechner-Scott, Jeannette
- Relation
- Clinical Epigenetics Vol. 7, Issue 118
- Publisher Link
- http://dx.doi.org/10.1186/s13148-015-0152-7
- Publisher
- BioMed Central
- Resource Type
- journal article
- Date
- 2015
- Description
- Background: Multiple sclerosis (MS) is thought to be a T cell-mediated autoimmune disorder. MS pathogenesis is likely due to a genetic predisposition triggered by a variety of environmental factors. Epigenetics, particularly DNA methylation, provide a logical interface for environmental factors to influence the genome. In this study we aim to identify DNA methylation changes associated with MS in CD8+ T cells in 30 relapsing remitting MS patients and 28 healthy blood donors using Illumina 450K methylation arrays. Findings: Seventy-nine differentially methylated CpGs were associated with MS. The methylation profile of CD8+ T cells was distinctive from our previously published data on CD4+ T cells in the same cohort. Most notably, there was no major CpG effect at the MS risk gene HLA-DRB1 locus in the CD8+ T cells. Conclusion: CD8+ T cells and CD4+ T cells have distinct DNA methylation profiles. This case–control study highlights the importance of distinctive cell subtypes when investigating epigenetic changes in MS and other complex diseases.
- Subject
- mulitple sclerosis; DNA methylation; CD8+ T cells; HLA-DRB1
- Identifier
- http://hdl.handle.net/1959.13/1330262
- Identifier
- uon:26346
- Identifier
- ISSN:1868-7083
- Rights
- This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Language
- eng
- Full Text
- Reviewed
- Hits: 2815
- Visitors: 3724
- Downloads: 660
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 509 KB | Adobe Acrobat PDF | View Details Download |